SAINBIOSE INSERM U1059Platforms

Cell Biology

Cell culture
  • NFS counter : MS4

MS4 reference in haematology. Allows small size, reliable and high-performance complete blood count within minutes. Clinical & preclinical.

  • Cell sorter : StemCell RoboSep

Fully automate your cell separation with the RoboSep™-S instrument. Using EasySep™ technology, RoboSep™-S performs all steps necessary to magnetically label and separate virtually any cell type by positive or negative selection. RoboSep™-S is designed to minimize sample handling, eliminate cross-contamination, and reduce hands-on time.

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Hemostasis
  • CS-2400

Automated instrument for Coagulation, with four simultaneous detection methods: chronometric assay, chromogenic kinetic analysis, immunoturbidimetric method and automated platelet aggregation.

  • Agregometer TA-4v (SD medical)

Semi-automates of platelet aggregation.

  • ROTEM

ROTEM® provides a graph representing Clot formation and lysis.

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Flow cytometry
  • Cytek Northern Light

The Cytek Northern Lights™ is a full spectrum flow cytometer with three laser system

    • localisation : Medical School
    • scientific manager : Pr. Stephane Paul
    • equipment manager :  Fabienne Jospin 
  • Cytoflex

The Cytoflex™ is a flow cytometer able to detect extracellular vesicles

  • Guava

The Guava® easyCyte systems use microcapillary & laser-based technology capable of detecting cells and beads.

  • BD Accuri C6

Accuri C6 is a 6-parameter benchtop cytometer. 2 Lasers 488 nm/ 640 nm and four filters. Acquisition software: BD CSampler.

  • Melody (cell-sorting)

2-ways cell sorter

    • localisation : Medical School
    • scientific manager : Pr. Stephane Paul
    • equipment manager :  Blandine Chanut
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Extracellular Vesicles analysis
  • Dynamic Light Scatter (Malavern)

Zetasizer DLS offers particle size, charge (zeta potential) analysis

  • NanoParticleTracking

NTA evaluate size and concentration of Extracellular Vesicles

  • Ultracentrifuge Beckman

XE-90 Beckman centrifuge

  • Rotor

SW-60 Ti

  • Rotors

SW-32 Ti and 42.2 Ti

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Metabolism
  • Fluoroskan Ascent Microplate Fluorometer

The Fluoroskan Ascent is used to measure fluorescence from 96-well microplates or other suitable formats in controlled incubation temperature with shaking and reagent dispensing capabilities.

  • Luminex MagPix

Multiplexing ELISA.Up to 50 analytes per sample.

  • Seahorse XF HS Mini analyzer

Seahorse Real-Time Cell Metabolic Analysis by Agilent. It measures and reports the oxygen consumption rate (OCR), proton efflux rate (PER) or extracellular acidification rate (ECAR), as well as ATP production rates of live cells in an eight-well miniplate format.

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Histology, histomorphometry,

Tissue preparation for quantitative bone histomorphometry (clinical & preclinical)
Imaging, quantitative bone histomorphometry (clinical & preclinical)
  • Microscope with motorized stage, Imascope image analysis software, interactive and semi-automatic measurements, 2 microscopes with UV fluorescence
  • Site: LBTO – Medical School
  • Scientific manager: Pr Marie-Hélène Lafage-Proust
  • Equipment manager: Norbert Laroche
Dicing Saw (Diamond Wire)

X-ray Imaging

Micro-computed tomography, preclinical research (vivaCT40, SCANCO Medical)
  • Mineralized tissue, and stained/infused soft tissue (10um resolution)
  • Site: LBTO Lab – Medical School
  • Scientific manager: Dr Marthe Rousseau
  • Equipment manager: Norbert Laroche
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Micro-computed tomography, preclinical research (Skyscan 1276, Bruker)
  • Coming soon!
  • The Bruker SkyScan 1276 desktop X-Ray Microtomograph is designed for fast, in vivo scanning of small lab animals and biological samples. The large field of view (up to 80 mm wide and 300 mm long) allows to scan larger items. High spatial resolution down to 6 μm voxel size and large image size are achieved by combining continuously variable magnification and 11 Mp X-ray CCD camera (2.8 μm pixel size). Other features include round and spiral scanning enabled by continuous gantry rotation, low dose imaging allowing longitudinal preclinical studies, and fast GPU-based 3D reconstruction from cross sections.
  • Site: LBTO Lab – Medical School
  • Scientific manager: Dr Marthe Rousseau
  • Equipment manager: Norbert Laroche
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Dual X-ray absorptiometry (DXA), preclinical research (PIXImus, Lunar)
  • Bone mineral density (BMD), with lean and fat mass content.
  • Site: LBTO Lab – Medical School
  • Scientific manager: Dr Marthe Rousseau
  • Equipment manager: Norbert Laroche

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Dual X-ray absorptiometry (DXA), clinical research
  • Bone mineral density (BMD), with lean and fat mass content.
  • Site: Rheumatology – University Hospital
  • Scientific manager: Pr Hubert Marotte
  • Equipment manager: Hervé Locrelle
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HRpQCT
  • Bone micro-architecture for hand/wrist/distal radius, and foot/ankle/distal tibia.
  • Site: Rheumatology – University Hospital
  • Scientific manager: Pr Hubert Marotte
  • Equipment manager: Hervé Locrelle
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Peripheral, quantitative tomography (pQCT)
  • Bone and mineral properties, any part of the body, with lean and at mass content.
  • Site: Rheumatology – University Hospital
  • Scientific manager: Pr Hubert Marotte
  • Equipment manager: Hervé Locrelle
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Microscopy

confocal microscopy
  • Confocal microscope (LEICA)  ZEISS LSM800 Airyscan
  • The Zeiss LSM800 with airyscan available room A124 is an inverted confocal laser scanning microscope with highly sensitive GaAsP detection and fast linear scanning. Airyscan acquire optical sections with a resolution of 120 nm (in x,y) and 350 nm (in z). With the 4-8x better signal-to-noise ratio (SNR) of Airyscan you will be pushing sensitivity and resolution. The Zen blue system is operating in a spectral mode, don’t worry about filters!! And the station is equipped with culture chamber for monitoring temperature CO2 and O2 pressure!
  • Site: LBTO Lab – Medical School
  • Scientific manager: Dr Alain Guignandon
  • Equipment manager: Arnaud Vanden-Bossche
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lightsheet microscope
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  • Lightsheet microscope (LEICA/EUR Manutech Sleight)
  • The Zeiss Lightsheet 7 for 3D imaging of live and cleared samples. This microscope provides fast and gentle imaging of living model organisms, tissues, and organoids as well as fixed optically cleared specimens. Specimens can be up to 2 cm in size and at any refractive index between 1.33 and 1.58 compatible with most clearing solutions.High quantum efficiency detectors enable observations of the fastest processes at the lowest illumination light levels. The light sheet geometry illuminates only the parts of the sample you are imaging, greatly reducing photobleaching and phototoxicity. You’ll get a real-life view of your samples without the adverse effects of excitation light on their biology.MAIN FEATURES:
    • Double side illumination objectives with single detection objective
    • Environmental module with CO2, humidity, and temperature control
    • Four imaging chambers for imaging live or fixed and cleared samples in broad range of sizes
    • Two PCO Edge 4.2 cameras with 16bit sCMOS sensor, 2048×2048 pixel density, 5µs to 5s exposure time, and maximum frame rate of 40fps at full resolution.
    • Four lasers 405nm, 488nm, 561nm, 638nm and fluorescence imaging channels (blue, green, red, and far red).
    • Advanced acquisition and analysis software operating of Lightsheet computer (Zeiss Zen Black- acquisition) and one high end workstations; (Zeiss Zen Blue, Arivis Vision PRO & VR). Zeiss Arivis Pro is modular image analysis software when working with multi-channel 2D, 3D, and 4D images of virtually unlimited size. This software can process large data sets of more than a Terabyte in size. Highly scalable computing, not dependent on local system resources. Processing and quantifying any kind of multidimensional image data. Combining different operators for complex analysis. Denoising, AI-driven segmentation, filtering, thresholding and more.
  • Site: LBTO Lab – Bat. Forges, Campus Manufacture
  • Scientific manager: Dr Alain Guignandon
  • Equipment manager: Arnaud Vanden-Bossche

Omics

Mass spectometry
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Transcriptomics
  • Nanostring

The nCounter® Sprint Profiler  combines preparation Station & Digital Analyzer.  Hybridized samples are loaded into a SPRINT cartridge, where a series of microfluidic chambers purifies and immobilizes the fluorescent Reporter Probes and readies them for imaging. Images are processed, and output files include gene names along with associated count numbers.

    • Site: Medical School
    • Scientific manager: Pr. Stéphane Paul & Mireille Paul
    • Equipment manager: Blandine Chanut 
  •  RNA quality/quantity and qPCR (TAPEstation 4150, Agilent) (CFX96, BioRad)

The 4150 TapeStation system is a low-throughput automated electrophoresis platform for nucleic acid sample quality control.

The CFX96 Touch System is a real-time PCR detection system. This six-channel (five colors and one FRET channel) real-time PCR instrument combines optical technology with precise temperature control to deliver sensitive detection for singlexplex or multiplex reactions.

  • 10X Genomics Chromium (coming soon)

The Chromium single cell gene expression provides single cell transcriptome 3′ gene expression and multiomics capabilities to profile tens of thousands of cells. Explore cellular heterogeneity, novel targets, and biomarkers with combined gene expression, cell surface protein expression, or CRISPR edits in each cell.

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Biomeca test plateform

Cardiovascular and Musculoskeletal Biomechanics

We combine mechanical testing (electroforce biaxial test bench, Instron uniaxial tensile testing machine, FSA pressure mapping system), optical systems (stereo-correlation bench with inflation test, fringe projection system, OCT microscope) and finite element software for biomedical applications including:

  • Mechanical characterization of soft tissues and textiles
  • Evaluation of medical devices and sport equipment
  • Numerical simulation
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Computational plateform

Centaur

Intensive computing at Mines de Saint-Étienne relies on high-performance computing infrastructures, in particular a computing cluster , named  Centaure (Rpeak = 41TFlops) composed of 28 CPU computing nodes for a total of 656 cores and 2, 5TB of RAM and  2 GPU compute nodes for a total of 4 GPU cards (a Tesla P100 and 3 amp A100). The characteristics of the front end and the calculation nodes are given below.

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Gravity - hyperG plateform

COMAT hyperG
  • Funded by the French Agency of Spatial Studies (CNES), this uniquely designed COMAT hyperG centrifuge measures 1.5m wide and is equiped with mobile gondolas and camera monitoring. This centrifuge can reach 2g, 3g, and up to 5g hypergravity. Rapid changes in gravity are possible, and efficiently mimic the phases of take-off and landing during spaceflights. The equipment allows vivo studies up to one month without discontinuation.

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